본문 바로가기

카테고리 없음

Advantages And Disadvantages Of The Serial Dilution Agar Plate Technique

Advantages And Disadvantages Of The Serial Dilution Agar Plate Technique

The idea behind 'plate tectonics' is that the surface of our Earth is composed of large 'plates' that move on a molten base. If the plates didn't move, the pressure from trapped heat would cause violent explosions.

Three dilutions (10-5, 10-6, 10-7) to duplicate nutrient agar plates, and label the plates. Spread the 0.1 ml inoculum evenly over the entire surface of one of the nutrient agar plates until the medium no longer appears moist. Return the spreader to the alcohol. Repeat the flaming and spreading for each of the remaining five plates. Serial dilution involves repeatedly mixing known amounts of source culture with (sterilised) liquid. 1 ml added to 9 ml gives a 10-fold dilution; 1 ml added to 99ml gives a 100-fold dilution. When fixed amounts of this dilution series are mixed with an appropriate agar and incubated, then different numbers of colonies will be obtained.

Advantages And Disadvantages Of The Serial Dilution Agar Plate Technique

For a small example, consider 'Old Faithful' in Yellowstone Park. It's a geyser - a sort of natural steam valve that releases the pressure of very hot subsurface water. For a more dramatic example, consider Krakatoa.

Advantages And Disadvantages Of The Serial Dilution Agar Plate Techniques

This was a huge volcanic eruption in Indonesia in 1883 that affected weather patterns all over the world. Of course, there are still problems where the plates meet. For example, the San Andreas fault, which runs under San Francisco, is actually a place where the North American and Pacific plates meet, and a little slipping along that fault line caused the great San Francisco earthquake in 1906. Because of the volcanoes formed by the subduction zones (where one tectonic plate falls and slides under an adjacent plate) gases from deep inside the earth are released. These gases probably formed our Nitrogen-Oxygen-Co2 atmosphere that is ideal for supporting life on our planet. In other areas where plates meet the plates push together and form non-volcanic mountains, providing variety in planetary ecosystems and species. The volcanoes also form new, extremely rich soil as in the Hawaiian Islands.

What Are Some Advantages And Disadvantages Of The Serial Dilution Agar Plate Technique

They also provide heat. Another benefit is that the mineral buildup in sea water and sea floor from continent erosion is lessened as the water and sea floor get recycled back into the molten mantle under the plates. Over many years this has helped provide a balance between minerals added and minerals subtracted. Another benefit is that metals from deep in the earth like copper are concentrated along these plate boundaries, making them easier to find and mine.

For more info, see the book Rare Earth.

Here's a paragraph about the molds that may help to infer the reason of the advantages during observation of mold colonies: 'Because the structural components of molds are very delicate, even simple handling with an inoculating loop may result in mechanical disruption of their components.The followi ng culture technique es used to to avoid this disruption. After culturing, molds spores are deposited in the surface of the agar and incubated in a moist chamber at room temperature. Direct microscopic observation is then possible without fear of disruption or damage to the anatomical components'.

It may be more beneficial in this aspect: allowing a full and healthy growth of the mold, that is, with its complete structure, then to be able to proceed to look through the microscope maybe it form, type of spore,sporangia or mycelium. In short, ensure that the mold to grow properly. Maybe it's not what you exactly were looking for but it could help you! Second Year of Microbiology bachelor's degree (University Of Puerto Rico in Arecibo) Paragraph taken of Capuccino/Sherman Microbiology. A laboratory Manual.Eight Edition.

The following are some advantages of an agar plate verses a slant tube: 1. Surface area- An agar plate has a much larger surface area: a. Easier to isolate individual colonies using the streak-plate method. Evaluate the colony shape, margin and elevation. Can grow a larger number of ce lls. Growth- An agar plate allows you to quantify the number of colonies on an agar plate, provided it is within the 30-300 range.

Whereas the slant tube cannot quantify growth but only describes growth as none, slight, moderate, or large. The idea behind 'plate tectonics' is that the surface of our Earth is composed of large 'plates' that move on a molten base. If the plates didn't move, the pressure from trapped heat would cause violent explosions. For a small example, consider 'Old Faithful' in Yellowstone Park. It's a geyser - a sort of natural steam valve that releases the pressure of very hot subsurface water. For a more dramatic example, consider Krakatoa. This was a huge volcanic eruption in Indonesia in 1883 that affected weather patterns all over the world.

Of course, there are still problems where the plates meet. For example, the San Andreas fault, which runs under San Francisco, is actually a place where the North American and Pacific plates meet, and a little slipping along that fault line caused the great San Francisco earthquake in 1906.

What Are Some Advantages And Disadvantages Of The Serial Dilution Agar Plate Technique (3 Pts)

Because of the volcanoes formed by the subduction zones (where one tectonic plate fall.

Advantages And Disadvantages Of The Serial Dilution Agar Plate Technique